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Determination of evodiamine and rutaecarpine in Evodia rutaecarpa and Huatuo Zaizao Pill by HPLC
To establish a quantitative method for the determination of two main active ingredients,evodiamine and rutaecarpine,in Chinese medicinal crop Evodia rutaecarpa and its preparation Huatuo Zaizao pill.METHOD:Evodiamine and rutaecarpine were determined by RP-HPLC for Evodia rutaecarpa,the mobile phase consisted of acetonitrile-water (50 50),the flow rate was 1.0ml.min-1ˇAmedroxyprogesteron acetate was used as internal standard.For Huatuo Zaizao pill,the mobile phase consisted of acetonitrile-water (40 60),the flow rate was 1.0ml.min-1,biphenyl was used as internal standard.The eluent was monitored with UV detector at 225nm.RESULTS:For Evodia rutaecarpa,the standard curves of evodiamine and rutaecarpine were all linear over the concentration range of 5-50μg.min-1.The average recoveries were 96.6%and 96.4%respectively.The intra-run relative standard deviations were 0.8%ˇH1.5%and 1.1%ˇH2.3%(n=6),respectively,the inter-run relative standard deviations were 2.3%ˇH3.6%and 2.5%ˇH3.9%(n=6),respectively.For Huatuo Zaizao pill,the standard curve of evodiamine and rutaecarpine were all linear over the range of 5-20μg*ml-1,the average recoveries were 97.6%and 94.6%,respectively.The contents of evodiamine and rutaecarpine in Evodia rutaecarpa from four localities of growth were 0.118%and 0.092%,0.213%and 0.106%,0.581%and 0.609%,0.478%and 0.459%,respectively.In Huatuo Zaizao pill,the contents of two ingredients were 0.1123mg.g-1 and 0.0981 mg.g-1,respectively.CONCLUSION:This method is accurate,convenient and sensitive.It is satisfactory for the quality control of Evodia rutaecarpa and its preparations. Treatment of 36cases with permanent atrial fibrillation with huatuo zaizao bolus
Progress in pharmacological and clinical studies on HUATUO ZAIZAO PILL
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