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Simultaneous determination of six Aconitum alkaloids in proprietary Chinese medicines by high-performance liquid chromatography
Ying Xiea, Zhi Hong Jianga, Hua Zhoua, Hong Xi Xub and Liang Liua, ,
aSchool of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong, People's Republic of China bHong Kong Jockey Club Institute of Chinese Medicine Limited, 2 Science Park East Avenue, Shatin, New Territories, Hong Kong, China
By optimizing the extraction, separation and analytical conditions, a reliable and accurate high-performance liquid chromatography (HPLC) method coupled with photodiode array detector (DAD) was developed for simultaneous quantitative determination of six Aconitum alkaloids, i.e., aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine, in Chinese medicinal herbs, aconite roots, and 12 proprietary Chinese medicines containing processed aconite roots. The separation of these Aconitum alkaloids was achieved on an ODS column with gradient elution using solvents of acetonitrile and ammonium bicarbonate buffer (pH 10.0 ± 0.2). Intra-assay and inter-assay precision of the analytes were less than 2.97%, and the average recovery rates obtained were in the range of 90–103% for all with RSDs below 3.28%. Good linear relationships of Xiao Huo Luo Wan were showed with correlation coefficients for the analytes exceeded 0.999. Quantitative analysis of the six Aconitum alkaloids in the unprocessed and processed aconite roots and in twelve proprietary Chinese medicines containing processed aconite roots showed that the contents of the alkaloids varied significantly. This method and quantitation results can provide a scientific and technical platform to the products manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary Chinese medicines and other herbal preparations containing aconite roots.
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