Experimental Study of Zhi Bai Di Huang Wan in Treating Ureaplasma Urealyticum and Enhancing Spermatozoa Motility
Journal of Liaoning College of Traditional Chinese Medicine 2007 Effects of successive cropping Rehmannia glutinosa on rhizosphere soil microbial flora and enzyme activities
Chen H, Hao HR, Xiong J, Qi XH, Zhang CY, Lin WX.
Institute of Agroecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
With the soils cropped Rehmannia glutinosa for one and two years as test materials, this paper studied the variations of microbial flora and enzyme activities in rhizosphere. The results showed that with increasing year of cropping, the numbers of bacteria and fungi in rihzosphere had a slight decrease. In the soil successively cropped R. glutinosa for two years, the number of actinomycetes in rihzosphere was 4 times higher, the ammonifier, aerobic azotobacter, sulphate reducer, denitrifier, and anaerobic cellulose-decomposer increased by 25.99, 45.39, 11.43, 1.36, and 1.43 folds, respectively, while aerobic cellulose-decomposer reduced by 86.74%, compared with those in the soil cropped R. glutinosa for one year. The root exudates of successively cropped R. glutinosa increased the activities of urease, polyhphenol oxidase, sucrase, protease and cellulase in rhizosphere by 62.87%, 9.43%, 47.91%, 139.62% and 31.33%, respectively, but inhibited the activity of catalase. The findings suggested that successive cropping R. glutinosa would destroy the balance of rhizosphere soil microbial flora.

Experimental Study of Zhi-Bai-Di-Huang Decoction in Treating Ureaplasma Urealyticum and Enhancing Spermatozoa Motility

The Effects of Extract of Zhi Bo Di Huang Wan on the [Ca2+]i of Rat Chromaffin Cell
Zhou Zhuan , Zhang Chunguang , Chen Liangyi , Ye Qin
By using microflurescent calcium measurement technique, the effects of extract of Zhi Bo Di Huang Wan on the intracellular calcium concentration ([Ca2+]i) of single rat Chromaffin cell were studied. The results showed the extract caused an increase of [Ca2+]i when it was puffing to the cell. Removing Ca2+ from the bath solution or nifedipine obviously inhibited the increasement of [Ca2+]i induced by the extract. It illustrated that the dependence of the Zhi Bo Di Huang-triggered calcium signal on extracellular Ca2+ and the opening of L-Ca2+ Channel made a great contribution to the [Ca2+]i increase.

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