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RAPD analysis on different varieties of Rehmannia glutinosa
Cheng JL, Huang LQ, Shao AJ, Lin SF.
Hospital of XuanWu Traditional Chinese Medicine, Beijing 100050, China.
OBJECTIVE: To provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa. METHOD: Nineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers. RESULT: The 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers, which divided the 19 varieties into four groups: Group Beijing, Group 85-5, Group Guolimao and the other Group. CONCLUSION: 8 varieties of Group Beijing have a close genetic relationship, and so have varieties of Group 85-5, which provides information for Rehmannia glutinosa's breeding.Ming mu di huang wan combined with electro-acupuncture for treatment of diabetic oculomotor paralysis in 52 cases.
The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, China.
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